Dear Daniel,
We used peptides (up to 40 aa long) from BioSynthesis, Lewisville, Texas
(www.biosyn.com) at 90% purity with great success. The material is
guaranteed to be 90% (or whatever you ask) pure but in reality it is a
lot better. (I have no commerical interest here myself). In one case we
even crystallized a complex with peptide that was only supposed to be
80% pure (and very nice electron density, crystals diffracting to 1.7 A).
Remy Loris
Vrije Universiteit Brussel
Daniel Jin schreef:
> Hi,
>
> We would like to order two peptides for co-crystallizaiton. I notice
> that there is a big price jump for peptide over 95% pure and over 98%
> pure. Do you think 95% purity is good enough? Another choice is to
> order crude peptides and purify by ourselves. Crude peptide is much
> cheaper. But we will then have to put in time to do multiple HPLC
> runs, reagent, cost for Mass Spec verification... Does anyone have
> suggestion about which one will be more efficient? Thanks.
>
> Best,
> Chen
>
>
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