Hi Alex,
Such gels were used to separate in the past 70S ribosome, plus/minus one
protein - so separation is excellent. Cannot recall the references but
papers published in the 70s' I believe.
The agaose is used to provide "strength" to low percentage acrylamide, say
3% acrylamide/0.5% agarose. Just need to cool the agarose before adding it
to the acrylamide.
Daniel
> Hello everyone:
>
> Sorry for the off-topic question. I am trying to separate by SDS-PAGE
> really big proteins (>500 kDa), and lower percentage (3-8%) acrylamide
> gels do not do the trick. Based on literature searches, acrylamide-agarose
> composite gels seem the way to go. Is anyone willing to share a protocol?
> I cannot get hold of any of the old journals where this was described at
> our rather limited library...
>
> Thanks a lot!
>
> Cheers,
> Alex
>
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