I'll second the recommendation for the Malvern Zetasizer. They are
rock-simple and interface with a computer through USB which makes future
computer upgrades relatively simple. The are however, not cheap--oops,
inexpensive.
Cheers,
--
------------------------------------------------------------------------
Roger S. Rowlett
Professor
Colgate University Presidential Scholar
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: [log in to unmask]
Andreas Förster wrote:
> Hey Thomas,
>
> also consider Malvern instruments. Their Zetasizers are really sweet
> and work with volumes smaller than 15ul if you use the smallest cuvette.
> http://www.malvern.com/LabEng/products/zetasizer/zetasizer.htm
>
> The last DynaPro that I've used, half as old as the universe but
> equipped with a 12ul cuvette, gave me really nice results also. Key is,
> as you discovered, to keep the cuvette meticulously clean. I used
> Pierce's RBS 35 Detergent for cleaning. Make a 2-5% dilution in a 50-ml
> beaker, heat to 70C in the microwave with the cuvette inside, let sit
> for a while, rinse with water and EtOH, and dry. Only touch with gloves
> afterwards.
>
> The protein sample must be spun down before the experiment. Half an
> hour at 13k in an Eppendorf centrifuge is sufficient. Make sure to
> avoid bubbles when adding the sample to the cuvette.
>
> Ah, nice data!
>
> Hope that helps.
>
>
> Andreas
>
>
> Thomas Edwards wrote:
>
>> Dear BB,
>>
>>
>>
>> Sorry for the off topic question:
>>
>>
>>
>> I would like to buy a Dynamic Light Scattering system.
>>
>> Could people suggest which they like the best and/or which is best value?
>>
>>
>>
>> I have in the past used a Protein Solutions Dyna Pro with micro cuvette
>> (I would like a micro cuvette option). However, it was very sensitive to
>> dust and bubbles, and the cuvette collects dust…
>>
>> I’ve never tried the one from Precision Detectors.
>>
>> Any other options?
>>
>>
>>
>> Thanks
>>
>> Ed
>>
>>
>>
>> ______________
>> T.Edwards Ph.D.
>> Garstang 8.53d
>> Astbury Centre for Structural Molecular Biology
>> University of Leeds, Leeds, LS2 9JT
>> Telephone: 0113 343 3031
>> <http://www.bmb.leeds.ac.uk/staff/tae/>http://www.bmb.leeds.ac.uk/staff/tae/
>> <http://www.bmb.leeds.ac.uk/staff/tae/Research>
>> "The doubter is a true man of science; he doubts only himself and his
>> interpretations, but he believes in science". ~Claude Bernard
>>
>>
>>
>>
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