...Or do the slightly-less-new-but-not-quite-old school technique:
QuickChange. Find a vector with roughly the properties you want, and
just quickchange
mutagenize in NdeI and ClaI sites at the desired positions. Works like
a charm
for me, assuming the vector is <10Kb or so, and I typically buy the various
components from the cheapest suppliers rather than the er... *cough*
$$$tratagene kit.*
Good luck,
D.S.
*-Though I'm sure their product is very nice... <shifty eyes>
Quoting Mark Collins <[log in to unmask]>:
> Or do the new school cloning, SLIC (Sequence & Ligation Independent
> Cloning) and "subclone" into any position in a vector.
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David M. Shechner, Graduate Student
Bartel Laboratory
Department of Biology
Whitehead Institute for Biomedical Research
Massachusetts Institute of Technology
[log in to unmask]
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