This is a job for the trusty Henderson-Hasselbach equation:
http://en.wikipedia.org/wiki/Henderson-Hasselbalch_equation
On Jul 21, 2008, at 8:12 PM, Meg wrote:
> Dear All,
>
> I want to prepare 25 mM sodium acetate buffer pH 4.5. can anyone
> give the
> exact composition of how to prepare it. we prepare it using sodium
> acetate
> and acetic acid combination. i am not able to arrive at the
> calculatation
> correctly, so if anyone can explain me with the above buffer how to
> calculate. and what sodium acetate [Anhydrous / trihydrate] and
> acetic acid
> [glacial/ plain] to use.
>
> thanks n regards
>
> Meg goyal,
> M.SC Biotechnology [Research]
> Institute of science,
> Fort
> Mumbai, INDIA
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