Why not get an in-line, flow-cell LS detector for use with your
chromatography system? We once had a great system, set up with a good SEC
column, UV detection at three wavelengths on an akta, followed by SLS and
DLS, and refractive index detector. The data were beautiful, as the SEC made
the background very clean, and one could see easily the degree of
mono/polydispersity of the SEC peak(s). It was not, however, inexpensive.
Jacob Keller
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [log in to unmask]
*******************************************
----- Original Message -----
From: "Gregor Witte" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Tuesday, July 08, 2008 10:27 AM
Subject: [ccp4bb] AW: [ccp4bb] DLS options?
Hmmm...
we had a demo of the Malvern Zetasizer instrument here, and to be honest: It
did not convince us at all (it is obviously built for non-biological
particle analysis)....
The Viscotek is also a plug&play device, every pc with a USB is suitable.
..in the end... Viscotek and Malvern are the same company now(!)
I guess the price for a DLS instrument is more or less independent of the
company.... aren't they all approx. 30-35k euros for a single-cuvette
system?
Gregor
-----Ursprüngliche Nachricht-----
Von: CCP4 bulletin board [mailto:[log in to unmask]] Im Auftrag von Roger
Rowlett
Gesendet: Dienstag, 8. Juli 2008 17:09
An: [log in to unmask]
Betreff: Re: [ccp4bb] DLS options?
I'll second the recommendation for the Malvern Zetasizer. They are
rock-simple and interface with a computer through USB which makes future
computer upgrades relatively simple. The are however, not cheap--oops,
inexpensive.
Cheers,
--
------------------------------------------------------------------------
Roger S. Rowlett
Professor
Colgate University Presidential Scholar
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: [log in to unmask]
Andreas Förster wrote:
> Hey Thomas,
>
> also consider Malvern instruments. Their Zetasizers are really sweet
> and work with volumes smaller than 15ul if you use the smallest cuvette.
> http://www.malvern.com/LabEng/products/zetasizer/zetasizer.htm
>
> The last DynaPro that I've used, half as old as the universe but
> equipped with a 12ul cuvette, gave me really nice results also. Key is,
> as you discovered, to keep the cuvette meticulously clean. I used
> Pierce's RBS 35 Detergent for cleaning. Make a 2-5% dilution in a 50-ml
> beaker, heat to 70C in the microwave with the cuvette inside, let sit
> for a while, rinse with water and EtOH, and dry. Only touch with gloves
> afterwards.
>
> The protein sample must be spun down before the experiment. Half an
> hour at 13k in an Eppendorf centrifuge is sufficient. Make sure to
> avoid bubbles when adding the sample to the cuvette.
>
> Ah, nice data!
>
> Hope that helps.
>
>
> Andreas
>
>
> Thomas Edwards wrote:
>
>> Dear BB,
>>
>>
>>
>> Sorry for the off topic question:
>>
>>
>>
>> I would like to buy a Dynamic Light Scattering system.
>>
>> Could people suggest which they like the best and/or which is best value?
>>
>>
>>
>> I have in the past used a Protein Solutions Dyna Pro with micro cuvette
>> (I would like a micro cuvette option). However, it was very sensitive to
>> dust and bubbles, and the cuvette collects dust.
>>
>> I've never tried the one from Precision Detectors.
>>
>> Any other options?
>>
>>
>>
>> Thanks
>>
>> Ed
>>
>>
>>
>> ______________
>> T.Edwards Ph.D.
>> Garstang 8.53d
>> Astbury Centre for Structural Molecular Biology
>> University of Leeds, Leeds, LS2 9JT
>> Telephone: 0113 343 3031
>>
<http://www.bmb.leeds.ac.uk/staff/tae/>http://www.bmb.leeds.ac.uk/staff/tae/
>> <http://www.bmb.leeds.ac.uk/staff/tae/Research>
>> "The doubter is a true man of science; he doubts only himself and his
>> interpretations, but he believes in science". ~Claude Bernard
>>
>>
>>
>>
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