Hi there,
I think 55% completeness is insufficient - you really need to collect more data.
Take a look at this movie from James Holton's website:
http://ucxray.berkeley.edu/~jamesh/movies/osc.mpeg
Stop the movie at 55% and check out how lousy the maps are compared to
95-100% - and I think these maps are generated with "perfect" phases,
at 1.5A resolution.
Molecular replacement programs and refinement programs are going to
struggle with just over half a dataset - this is also why your
R-factors are sky high.
In addition, I know of cases where reviewers have cast doubt over
structures (of a point mutant) with data of 80% completeness. I don't
think a 55% complete dataset is going to get out there.
I really think more data is the only way our of your predicament.
I hope you have a few crystals ready to go!
Good Luck,
David.
2008/7/27 Carl Soja <[log in to unmask]>:
> HI all,
>
> Thank you very much for your kindly answer.
>
> I also tried to use different program like phaser, amore, and some MR servers.
> actualy, I don`t know how many molecule in AU clearly. 2-6 molecules in 76%-
> 35% of solvent content. 4mer is 50% of solvent content. the homolgous
> structure has high solvent content over 70%. when i used phaser program to
> do MR, I can get several solutions but not good structure conformation. I
> don`t know how to use phaser do automatic more than 2 molecules search
> like molrep program.
> As spacegroup, I checked the C222 and C2221. the phaser program can give
> both solutions when I changed the search model or maximum solution limit.
>
> my question is how i can search more than 2 molecules by phaser program like
> molrp only input over 2.
>
> by the way, my diffraction data have low completeness(55%) and low
> redandancy(1.8).
>
> Thanks in advance!!
>
> carl roja
>
--
============================
David C. Briggs PhD
Father & Crystallographer
http://www.dbriggs.talktalk.net
AIM ID: dbassophile
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