Dear All:
I am working with two enzymes (40 kDa each). The crystal structures of
the two enzymes are known.
I have made a hybrid enzyme of the two individual ones.
This chimeric/hybrid enzyme is soluble and exhibits activity of the two
individual enzymes.
My questions are as follows:
1. Could I use one of the enzyme's structure to solve the crystal
structure of the hybrid by MR?
2. Has anyone working with such hybrids (?) noticed any increase in
specific activity of either of the enzymes?
Interestingly, the two enzymes work on the same substrate polymer. One
is a hydrolase another is an esterase.
Thanks a lot.
Subbu
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