I've been making some titrations lately and have run into some trouble
analysing the data.
1) When I make histograms of resonance1 vs shift dist The resonances are not
displayed on the x axis, but they are for other people in the lab. Still we
are all using the same installation of Analysis. See attachment for screenshot.
2) When I try to fit my titration to the saturation curve function in
analysis (the longest function, 2nd from bottom) and then press group peaks
I get a error (see screen dump). The annoying thing is that I got it to work
with another titration 2 weeks ago, but not this time. I've tried both
ligand ratio and ligand conc.
3) When I managed to fit the function 2 weeks ago I got a really nice
fitting curve and equation (with the variable values). Theoretically, I
should be able to derive the Kd from this function. It would be really nice
to be able to do that right away in Analysis rather than using some other
program. Anyhow, I don't recognize the equation and I find it strange that
it doesn't need the protein concentration, just the ligand. Do you have some
kind of reference on this equation where I can read about it, or where does
it come from? Do you know how I can get the Kd from the equation?
Thanks
Conny
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