Hi everyone,
can anyone tell me the protocol to revive human corneal cell lineor any
cell line which requires serum free media
i usually used serum based media initially.....later switched it to serum
free media for subculturing....only in case of human corneal cell line
with the help of flask coated with fibronectin, BSA, and
collagen..........but recently i m finding difficult to revive it....it
deattaches after 1 or 2 days..what might be the reason? also we are unable
to revive pRSV-T cell line using flask coating method. can anyone help on
the same.
thanks
|