Hi Ji,
Our lab has had good luck using sodium malonate to cryoprotect salt-
grown crystals. See:
Acta Cryst. (2003). D59, 2356-2358
Malonate: a versatile cryoprotectant and stabilizing solution for salt-
grown macromolecular crystals
T. Holyoak, T. D. Fenn, M. A. Wilson, A. G. Moulin, D. Ringe and G. A.
Petsko
Best,
Wally
On Jun 24, 2008, at 2:14 AM, Ji lee wrote:
> Dear,
>
> I have a crystal diffracted anisotrophically. I tested with a few
> different cryo conditions like oil, glycerol in different
> concentration to get a better data but these conditions didn't help
> any.
> Using capillary method improved the diffraction (isotrophic) but the
> crystal couldn't survive during the data collection.
> Is there any methods or cryo conditions I can use to improve my
> diffraction data?
> This crystal grew in 2.5M Ammonium Sulfate.
>
> Thank you so much.
>
> Ji
Walter R.P. Novak, Ph.D.
Postdoctoral Fellow
Rosenstiel Basic Medical Research Center
Brandeis University
415 South St. MS 029
Waltham, MA 02454-9110
Phone: (781) 736-4944
Fax: (781) 736-2405
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