dear john,
the reference for this in my opinion is still the 1994 paper by george and wilson where they describe the use of the second virial coefficient parameter as a metric for quantifying the potential that certain conditions have to favour crystallisation.
see for instance bonete and vivares, Acta Cryst. (2002). D58, 1571-1575.
cheers,
jose brandao
________________________________
From: CCP4 bulletin board on behalf of John Luz
Sent: Mon 23/06/2008 21:14
To: [log in to unmask]
Subject: [ccp4bb] RI and MALS for membrane proteins
Would like to know what experiences people have had using in line refractive index and multiangle light scattering detectors for membrane protein purification. Specifically, if the information gathered from such experiments has been useful in optimizing solution conditions for membrane protein preparations to be used in crystallization trials. Thanks in advance for any replies.
j
John Gately Luz
Snr. Scientist
Protein Biochemistry
SGX Pharmaceuticals, Inc.
San Diego, CA
phone: 858-228-1482
fax: 858-457-5362
email: [log in to unmask]
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