Hi Gina,
From a lab near you ... gd resolvase without DNA: a
hexagonal crystal form can be grown from either the
catalytic domain only (missing the DNA binding domain, ~1/3
of the protein) or the intact protein. The binding domain
is there when you run crystals down a gel, but disordered in
the rather lousy, low-resolution, anisotropic electron
density maps.
Phoebe
Rice PA, Steitz TA.
Model for a DNA-mediated synaptic complex suggested by
crystal packing of gamma delta resolvase subunits.
EMBO J. 1994 Apr 1;13(7):1514-24.
Abdel-Meguid SS, Murthy HM, Steitz TA.
Preliminary X-ray diffraction studies of the putative
catalytic domain of gamma delta resolvase from Escherichia
coli. J Biol Chem. 1986 Dec 5;261(34):15934-5.
---- Original message ----
>Date: Sat, 28 Jun 2008 18:30:38 -0400
>From: Gina Clayton <[log in to unmask]>
>Subject: [ccp4bb] Disordered domains in crystal strutures
>To: [log in to unmask]
>
>Dear CCP4ers
>
>can anyone recommend papers describing crystal structures
of proteins
>with a large functionally important disordered domain or
domains.
>
>Thanks in advance
>
>Gina
Phoebe A. Rice
Assoc. Prof., Dept. of Biochemistry & Molecular Biology
The University of Chicago
phone 773 834 1723
http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123
RNA is really nifty
DNA is over fifty
We have put them
both in one book
Please do take a
really good look
http://www.rsc.org/shop/books/2008/9780854042722.asp
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