parkash wrote:
> Hi,
> I have one structural refinement problem.
> I am working on a protein crystals which diffracted to 2.8 Å. But when
> I refine through REFMAC5, with 0.1 wt(geometry to x-ray terms), I get
> high B-factors around 70. But if I do TLS refinement, the R-factors
> lower down and B-factors come down to 40.
>
> My question is; Are TLS refined B-factors real?? Can I trust them?
> or what else could be done to lower down the B.factors?
>
> BR
> vimal
>
>
>
This is or should be a FAQ - all B factors after TLS are relative to the
TLS restraints
Try running TLSANL and that should give you back a more meaningful
overall B
Eleanor
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