Dear Andrew,
The following paper actually measured the increase in lifetime offered
to the crystal if data are collected at 100K instead of room temperature:
Southworth-Davies, R. J., Medina, M. A., Carmichael, I. & Garman, E. F.
(2007) Observation of decreased radiation damage at higher dose rates in
room temperature protein crystallography. Structure. 15, 1531-41.
The gain in lifetime (factor of 26 - 113) is similar to the value
(factor 70) estimated in:
Nave, C. & Garman, E. F. (2005) Towards an understanding of radiation
damage in cryocooled macromolecular crystals. J Synchrotron Radiat 12,
257-60.
Best regards,
Martin
Andrew Torelli a écrit :
> To the CCP4 community,
> I believe I have identified 2 appropriate citations for the use of
> cryocooling to mitigate the effects of radiation damage during a
> diffraction experiment. The first study is one that I have access to:
> Low, B. W., Chen, C. C., Berger, J. E., Singman, L. & Pletcher, J. F.
> (1966). Proc. Natl Acad. Sci. USA, 56, 1746–1750.
> The second study is one that I cannot find text for:
> Blake, C. F. F. & Phillips, D. C. (1962). Biological Effects of
> Ionizing Radiation at the Molecular Level, pp. 183–191. Vienna: IAEA.
> Could someone point me in the right direction to find this text?
> Alternatively, are there other citations that people use for the
> general use of cryocooling crystals in X-ray crystallography?
> Thank you for your time,
> Regards,
> -Andy Torelli
|