Narayanan Ramasubbu a écrit :
> Dear all:
> I am noticing that in some of my structures, at 1.5 A resolution, 1)
> there is some negative density around the C of the carboxyl groups.
> 2) I also notice the negative density around S in a disulfide region.
> It is as though the disulfide is broken.
>
> Could some body enlighten me on this feature?
>
>
>
The other original reference for the radiation damage effect on carboxyls
is actually:
Burmeister, W. P.
Structural changes in cryo-cooled protein crystals owed to radiation damage.
Acta Cryst. D 56, 328-341 (2000).
Greetings
Wim Burmeister
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Wim Burmeister
Professeur, Membre de l'Institut Universitaire de France
Unit of Virus Host Cell Interactions (UVHCI) UMR5233 UJF-EMBL-CNRS
6 rue Jules Horowitz
B.P. 181, F-38042 Grenoble Cedex 9 FRANCE
E-mail: [log in to unmask]
Tel: +33 (0) 476 20 72 82 Fax: +33 (0) 476 20 94 00
http://www2.ujf-grenoble.fr/pharmacie/laboratoires/gdrviro
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