Hi, with S2 you can get away without figuring out if the virus works, then
again you probably need a stable cell line... (so which ever works...?)
(OBS! cant do SeMet labelling with S2!! or if someone can please tell me)
-Tommi
Quoting Yong-Fu Li <[log in to unmask]>:
> Hi,
>
> I searched my mail box for possible answers you have given but couldn't
> find
> any. The question is about selecting insect expression systems for
> mammalian
> or viral glycosylated proteins. There is confirmed expression of the
> target
> proteins in mammalian cells. They are secreted into the media.
>
> 1) Baculovirus system
> 2) Drosophila system
>
> Which system would you recommend for high expression and convenience?
> Has anyone ever compared those systems side by side?
>
> Any suggestions or references are greatly appreciated.
>
> Yongfu Li
>
--
Tommi Kajander, Ph.D.
Macromolecular X-ray Crystallography
Research Program in Structural Biology and Biophysics
Institute of Biotechnology
P.O. Box 65 (Street address: Viikinkaari 1, 4th floor)
University of Helsinki
FIN-00014 Helsinki, Finland
Tel. +358-9-191 58903
Fax +358-9-191 59940
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