You can try:
http://toolkit.tuebingen.mpg.de/hhpred
this gives you a nice domain border prediction based on analog. structures.
Best wishes
Kornelius
On Tue, 20 May 2008 19:34:39 +0200
Jayashankar <[log in to unmask]> wrote:
> Dear friends and scientists,
>
> (A pre-Structural biological question.)
>
> I Have a multidomain protein , I know the domain boundaries,
> But am still not that rational to what residues a construct should start or
> end?
>
> But I have learned from people that changing one residue changes the fate of
> the construct.
>
>
> I will be greatful for the insights from the scientific community.
> I dont want to do any Directed evolution approach.
>
> --
> S.Jayashankar
> Research Student
> Institute for Biophysical Chemistry
> Hannover Medical School
> Germany
----------------------------------------------
Kornelius Zeth
Max Planck Institute for Developmental Biology
Dept. Protein Evolution
Spemannstr. 35
72076 Tuebingen, Germany
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Tel -49 7071 601 323
Fax -49 7071 601 349
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