Hi,
I have one structural refinement problem.
I am working on a protein crystals which diffracted to 2.8 Å. But when I
refine through REFMAC5, with 0.1 wt(geometry to x-ray terms), I get high
B-factors around 70. But if I do TLS refinement, the R-factors lower
down and B-factors come down to 40.
My question is; Are TLS refined B-factors real?? Can I trust them?
or what else could be done to lower down the B.factors?
BR
vimal
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