I had the similar problems with the protein expression but I tried heat
shock at 42 degree C for 25 mins before induction with IPTG and
approximately 60% of the overexpressed protein become soluble. The
explanation for this is the upregulation of heat shock proteins that may
help in the refolding of the overexpressed proteins. So it is woth to try.
Best of luck.
Mohinder
|