Does this include the customary grieving period?
On Apr 14, 2008, at 2:10 PM, JOE CRYSTAL wrote:
> Hi,
>
>
> Does anyone have information about how long it takes to set up a 96-
> well
> tray for the crystallization robots available? Besides cost per
> tray and
> maintenance cost, another important feature we consider is the time
> for
> setting up a 96-well tray. It is an important factor since we are
> talking
> about sub-microliter drops.
>
>
> Best,
>
>
> Joe
>
> On Fri, Jan 18, 2008 at 12:28 PM, Lisa A Nagy <[log in to unmask]>
> wrote:
>
>> Al's Oil on the plates:
>> What a nightmare!!!!!!!
>> The oil creeps up the plate and over the sides. It dissolves
>> adhesives.
>> It makes me say bad words in multiple languages.
>> Bigger drops + no oil = fewer bad words.
>>
>> Lisa
>> --
>> Lisa A. Nagy, Ph.D.
>> University of Alabama-Birmingham
>> [log in to unmask]
>>
>> -----Original Message-----
>> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
>> Patrick Shaw Stewart
>> Sent: Friday, January 18, 2008 2:20 AM
>> To: [log in to unmask]
>> Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
>>
>> One thing that people often overlook is that quite a lot of protein
>> can be lost by denaturation on the surface of the drop. This is more
>> significant for smaller drops. Two suggestions: (1) increase the
>> proportion of protein in the - technical term - teeny drop to say two
>> thirds and (2) cover the drops with oil eg Al's oils
>> (silicone/paraffin). You still get vapor diffusion though the oil ,
>> and you'd like to slow up equilibration. of course (2) slows up the
>> robotics a little, but both should be trivial to set up..
>>
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