Lower temperature, use chaperones (e.g. TAKARA set), refolding?
Quoting shivesh kumar <[log in to unmask]>:
> Dear all,
> Sorry for the off-topic question...
> What can be done to avoid a protein going inside inclusion body.The gene is
> cloned in pET30a with C-ter his tag and expressed in BL21-DE3 from 37 to
> 18C for 3-4 hr with .5mM of IPTG,it is going to inclusion body.All
> suggestions are welcome.
> Thanx in advance.
> Shivesh
>
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