We normally use AmS or glycerol.
Poul
On 07/04/2008, at 15.46, Kay Diederichs wrote:
> Dear all,
>
> a protein which we work on is available in low quantity. The only
> crystallization screen we set up is completely clear, no
> precipitate, nothing.
>
> Now we would like to modify the reservoirs of this screen, by adding
> LiCl or Ammoniumsulfate or ... , with the goal of reducing the
> vapour pressure, to at least get the protein concentration in the
> drop into the range where "something happens".
>
> Does anyone have advice as to which salt we should add (to the
> reservoir only)? AmSO4 is only soluble to 4M, LiCl goes to 10M. But
> vapour pressure reduction is not the same as molarity.
>
> thanks for any insight,
>
> Kay
> --
> Kay Diederichs http://strucbio.biologie.uni-konstanz.de
> email: [log in to unmask] Tel +49 7531 88 4049 Fax 3183
> Fachbereich Biologie, Universität Konstanz, Box M647, D-78457 Konstanz
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