This might help...
Hassell AM, An G, Bledsoe RK, Bynum JM, Carter HL 3rd, Deng SJ, Gampe
RT, Grisard TE, Madauss KP, Nolte RT, Rocque WJ, Wang L, Weaver KL,
Williams SP, Wisely GB, Xu R, Shewchuk LM.
Abstract
Crystallization of protein-ligand complexes.
Acta Crystallogr D Biol Crystallogr. 2007 Jan;63(Pt 1):72-9. Epub 2006
Dec 13. Review.
PMID: 17164529 [PubMed - indexed for MEDLINE]
hth
Dave
On 16/04/2008, Jiamu Du <[log in to unmask]> wrote:
> Dear All,
> I want to crystallize a mAb Fab in complex with a hydrophobic cyclic
> peptide. The peptide contains 1 hydrophilic residue, 2 Cys, and 9
> hydrophobic residues. The problem is that I can not dissolve this peptide in
> common buffers. I think it is need to dissolve it in some organic solvent,
> such as DMSO. But organic solvent is harmful to protein.
> Is there any sugestions for this situation?
>
> Thanks.
>
> --
> Jiamu Du
> State Key Laboratory of Molecular Biology
> Institute of Biochemistry and Cell Biology Shanghai Institutes for
> Biological Sciences
> Chinese Academy of Sciences (CAS)
--
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David C. Briggs PhD
Father & Crystallographer
http://www.dbriggs.talktalk.net
AIM ID: dbassophile
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