We also have this machine (Cartesian Honeybee X8 ) and can verify that
dispensing 3 drops per condition increases the overall build time about
30 seconds.
We have also recently acquired a Cartesian Honeybee 963 which will do
the whole experiment setup, including dispensing precipitant into the
well and onto the shelf, in 6 minutes for 2 protein sites (including all
washes). A 3 site experiment would take 30 seconds longer. The actual
time from first drop dispense to last is about 1 minute.
Stephen
SSPF
University of St Andrews
> We use Cartesian Honeybee X8 machines (8 tips). They take about 10 minutes to set a 96-drop plate including the washes of the tips. 3 or 4 drops per condition wouldnt take much longer. Optimisation and additive/detergent screens take a little less time.
>
> The plates are pipetted under a close-fitting cover to (virtually) eliminate evaporation, which IMO is better than a humidity chamber. Consumable costs extend to isopropanol and water, with the occasional replacement valve or tip.
>
> Since people here also tend to turn up at beer o'clock on a Friday evening (must be an Oxford thing...) we have two machines (and another one imminent) to increase throughput.
>
> HTH
> Tom
>
> ** Tom Walter B.Sc. M.Res. **
> ** Oxford Protein Production Facility Tel: +44 (0)1865 287747 **
> ** Wellcome Trust Centre for Human Genetics Fax: +44 (0)1865 287547 **
> ** Roosevelt Drive [log in to unmask] **
> ** Headington, Oxford OX3 7BN http://www.oppf.ox.ac.uk **
>
>
> ---- Original message ----
>
>> Date: Mon, 14 Apr 2008 17:10:26 -0400
>> From: JOE CRYSTAL <[log in to unmask]>
>> Subject: Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
>> To: [log in to unmask]
>>
>> Hi,
>>
>> Does anyone have information about how long it takes to set up
>> a 96-well tray for the crystallization robots available?
>> Besides cost per tray and maintenance cost, another important
>> feature we consider is the time for setting up a 96-well
>> tray. It is an important factor since we are talking about
>> sub-microliter drops.
>>
>> Best,
>>
>> Joe
>>
>> On Fri, Jan 18, 2008 at 12:28 PM, Lisa A Nagy
>> <[log in to unmask]> wrote:
>>
>> Al's Oil on the plates:
>> What a nightmare!!!!!!!
>> The oil creeps up the plate and over the sides. It dissolves
>> adhesives.
>> It makes me say bad words in multiple languages.
>> Bigger drops + no oil = fewer bad words.
>> Lisa
>> --
>> Lisa A. Nagy, Ph.D.
>> University of Alabama-Birmingham
>> [log in to unmask]
>>
>> -----Original Message-----
>> From: CCP4 bulletin board [mailto:[log in to unmask]] On
>> Behalf Of
>> Patrick Shaw Stewart
>> Sent: Friday, January 18, 2008 2:20 AM
>> To: [log in to unmask]
>> Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
>>
>> One thing that people often overlook is that quite a lot of
>> protein
>> can be lost by denaturation on the surface of the drop.
>> This is more
>> significant for smaller drops. Two suggestions: (1)
>> increase the
>> proportion of protein in the - technical term - teeny drop
>> to say two
>> thirds and (2) cover the drops with oil eg Al's oils
>> (silicone/paraffin). You still get vapor diffusion though
>> the oil ,
>> and you'd like to slow up equilibration. of course (2)
>> slows up the
>> robotics a little, but both should be trivial to set up..
>>
>
>
>
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