Hello.
I've been running an experiment that is basically a hCC-NcH-TOCSY. In
the experiment the 13C-shifts of the sidechain is correlated with the
sidechain 15N-shifts of lysines (~33 ppm) and arginines (~79 ppm) and
the neighbouring 1H-shifts (Hdelta and Hepsilon).
I process my spectra in nmrPipe and import them as UCSF-format.
As reference experiment I choose hC_cNH.TOCSY, which is the closest
match to the actual experiment, I believe.
The problems starts when I try to view the spectra in CCPNMR. In the
1H-15N window (with 13C as z-axis) I cannot go below 80 ppm and since my
reference ppm is 58.3, I can't see anything.
Is there a way to shift the 1H-15N spectra to show spectra that are not
located in the normal amide backbone range?
In the 1H-13C window (with 15N as z-axis), I dont' see anything even at
basically zero contour level, even if get the dotted square indicating
my spectral window. If I activate sidetraces it is possible to view the
intensities as 1D-slices and they seem OK. Have I chosen the wrong type
of reference experiment? How do you create your own custom experiment?
Regards
--
Carl Diehl
Department of Biophysical Chemistry
Lund University
046-2220384
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