Dear All,
An alternative view would be that you should use Hba if you are not sure
how many protons you have, and only use Hb* when you are sure you have
both of them there.
Anyway, we are considering getting rid of Hb* altogether, since it seems
to be more trouble than it is worth.
What do people think?
Yours,
Rasmus
---------------------------------------------------------------------------
Dr. Rasmus H. Fogh Email: [log in to unmask]
Dept. of Biochemistry, University of Cambridge,
80 Tennis Court Road, Cambridge CB2 1GA, UK. FAX (01223)766002
On Wed, 5 Mar 2008, Christoph Brockmann wrote:
> Hi there,
>
> I've just noticed that the assignment of Hb* defaults to the assignment
> of Hb2 and Hb3 even when there are no other stereo-specific assignments.
> This causes more head-ache than necessary, should it turn out later that
> there is a second Hb resonance and one has to resolve this to Hba and Hbb.
>
> In my personal opinion, everything should default into
> non-stereospecific assignments unless explicitly told otherwise. (In
> case of degenerate Hb, this should make no difference anyway).
>
> How do others feel about this issue?
>
> Christoph
>
> --
> ---------------------------------------------------------
> Dr. Christoph Brockmann
> Division of Structural Studies
> MRC Laboratory of Molecular Biology
> Hills Road
> Cambridge, CB2 2QH
> phone: +44-1223-40-2261 or +44-1223-40-2042
> e-mail: [log in to unmask]
> ---------------------------------------------------------
>
|