Hi
I'm probably missing something very obvious here, but I have been attempting to run DM,
specifically averaging, for my particular case (3 mol/asym. unit, a MBP fusion protein, so I'm
expecting something extra).
I have entered the rotation / translation operators in the CCP4i menu, and I believe that I've
provided the right files, etc. However, I get an error:
has failed with error message
dm: (RCARDS) AVER: enter indentity matrix first
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although the first operator I entered under the "domain number 1" subheading is the identity
operator, with the identity matrix and no Cartesian coordinate transformation.
Incidentally, how many operators do I need to enter? I have the B -> A transformation, and the C
-> A transformation, I think that's everything.
Related question, the density for my fusion protein is hard to grasp, although naturally the MBP
portions look relatively nice. What would be your criterion for disordered density, in essence,
when would you 'give up' and chalk it up to disorder, vs. weak density, vs. multiple
conformations?
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