Quoting James Stroud <[log in to unmask]>:
> Try refolding before purification.
OR, you could do a denaturing purification, followed by refolding. Ni-NTA's
perfectly viable in denaturing urea, for example. From there, it's just a
question of whether to try refolding on-resin, or following elution.
Cheers,
d.s.
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David M. Shechner, Graduate Student
Bartel Laboratory
Department of Biology
Whitehead Institute for Biomedical Research
Massachusetts Institute of Technology
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