If your protein is autolyzing, you may need to crystallize it in the
presence of an inhibitor. In the case of trypsin, I have crystallized
in the presence of benzamidine, then removed the inhibitor by dialysis
after crystallization.
kmj
Ngo Duc Tri wrote:
> Dear CCP4 experts,
> I'm working on a kind of cystein peptidase. It shows a little
> degradation after 2 days storing at 4 degree even I tried many kinds of
> protease inhibitors, or glycerol and DTT.
> So I decided to work fast (1 day) then set xtal. After one week I pick
> up some condition and check degradation by SDS-PAGE. It shows heavy
> degradation then I think it's impossible to obtain the crystal if I
> don't find out how to stop the degradation first.
>
> Do you have any idea about my situation? Is it true that I need to stop
> the degradation and how to deal with it?
> Thank you for your advices,
>
> My best regards,
> TriNgo
>
>
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