On Mar 12, 2008, at 12:48 PM, Jan Schoepe wrote:
> Hello everybody,
>
> I wonder if anybody has experience with heme (or to be more precise:
> heme b) containing proteins which Xtals do not look red under the
> microscope. How might the technique for crystallization (e.g.
> sitting drop, hanging drop) influence the intensity of the color?
> Many thanks!
If there is no metal in the heme, its color will be very different.
If your crystallization buffer contains EDTA or other strong chelating
agents, I imagine this might happen, though protein crystallization is
not my area.
Ian
|