Dear Carl,
The ExperimentPrototype you want is now checked in and available on the
update server. It is called 'HC_C[N]H.TOCSY. It would be nice if you could
go into the editor and take a look, just to make sure I did not
misunderstand your experiment.
Cheers,
Rasmus
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Dr. Rasmus H. Fogh Email: [log in to unmask]
Dept. of Biochemistry, University of Cambridge,
80 Tennis Court Road, Cambridge CB2 1GA, UK. FAX (01223)766002
On Mon, 3 Mar 2008, Carl Diehl wrote:
> Hello.
> I've been running an experiment that is basically a hCC-NcH-TOCSY. In
> the experiment the 13C-shifts of the sidechain is correlated with the
> sidechain 15N-shifts of lysines (~33 ppm) and arginines (~79 ppm) and
> the neighbouring 1H-shifts (Hdelta and Hepsilon).
> I process my spectra in nmrPipe and import them as UCSF-format.
>
> As reference experiment I choose hC_cNH.TOCSY, which is the closest
> match to the actual experiment, I believe.
>
> The problems starts when I try to view the spectra in CCPNMR. In the
> 1H-15N window (with 13C as z-axis) I cannot go below 80 ppm and since my
> reference ppm is 58.3, I can't see anything.
> Is there a way to shift the 1H-15N spectra to show spectra that are not
> located in the normal amide backbone range?
>
> In the 1H-13C window (with 15N as z-axis), I dont' see anything even at
> basically zero contour level, even if get the dotted square indicating
> my spectral window. If I activate sidetraces it is possible to view the
> intensities as 1D-slices and they seem OK. Have I chosen the wrong type
> of reference experiment? How do you create your own custom experiment?
>
> Regards
>
> --
> Carl Diehl
> Department of Biophysical Chemistry
> Lund University
> 046-2220384
>
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