> At least that's been our experience.
> Andy
> (No affiliation with NEB)
Hi, everyone:
Just to give both sides to this story, though, we've had phenomenally bad luck
with the NEB Intein system (tagged on either the C- or N- terminus). The
protein expressed and purified beautifully, and post-cleavage was
pretty much a
single band on a silver-stained gel. However, it was intractably misfolded,
while purification with other, more 'traditional' tags (His6 or - dare I say
it - *untagged* purification!) yielded fully soluble, folded protein. A few
other labs around MIT have had similar experiences. So, for any technique
there will be circumstantial data affirming or refuting its utility; take any
success or failure story with the required ug of NaCl. =)
Good luck purifying,
Dave
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