Kornelius,
have a look at the following paper:
Yeh AP, McMillan A, Stowell MH.
<http://www.ncbi.nlm.nih.gov/pubmed/16552147?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum>
<http://www.ncbi.nlm.nih.gov/pubmed/16552147?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum>
Rapid and simple protein-stability screens: application to membrane
proteins.
Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):451-7. Epub 2006
Mar 18.
- J. -
Kornelius Zeth wrote:
> Dear all,
>
> a question very related to the discussion before. I have been reading the papers about the thermofluor experiment with great interest.
>
> I wonder what people think about the underlying principles/ideas and the success that the method yielded in their own labs for crystallization or related purposes?
>
> Has anybody used this method with membrane proteins in order to find out the stability of the protein in the presence of a second detergent?
>
> Is the method limited to this certain dye (sypro orange)?
>
> Have a nice day
>
> Kornelius
>
> P.S.: I will make a summary of all opinions.
>
> ----------------------------------------------
> Kornelius Zeth
> Max Planck Institute for Developmental Biology
> Dept. Protein Evolution
> Spemannstr. 35
> 72076 Tuebingen, Germany
> [log in to unmask]
> Tel -49 7071 601 323
> Fax -49 7071 601 349
>
--
Jeroen Raymundus Mesters, Ph.D.
Institut fuer Biochemie, Universitaet zu Luebeck
Zentrum fuer Medizinische Struktur und Zellbiologie
Ratzeburger Allee 160, D-23538 Luebeck
Tel: +49-451-5004070, Fax: +49-451-5004068
E-mail: [log in to unmask]
Http://www.biochem.uni-luebeck.de
Http://www.iobcr.org
Http://www.opticryst.org
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