> 1) In my current project I have defined three chains within one
> molecular system, each of which is the same polypeptide chain, but in a
> different conformation (i.e. different shifts). Would it be possible to
> assign a specific cross-peak to a particular amino acid (e.g. Leu-15)
> while keeping it uncertain which 'chain' it belongs to? It is often
> (initially) easier to assign sections than it is to figure out which
> sections belong to which chain (conformation).
The offical way to do this will appear once Analyis can handle the
"ChainStates" within the CCPN data model. However, the implementation of
this is some time away.
At present you could put a text label on the peaks M::Peak::Set Details.
(And text labelling of spin systems can potentially be added too)
> 2) In terms of assigning those subsections: the 'Link Seq Spin Systems'
> dialog seems designed for that purpose, but seems to require specific
> solution NMR experiments to be present before it can work. In SSNMR the
> backbone assignment approaches give the same information, but they don't
> seem to 'work' with this dialog box.
I will enable the solid state experiments if you can let me know which
experiments (of pairs therof) would commonly be used.
In this regard I have also had a request for the through carbonyl side
alpha/beta 1H experiments.
> The only thing I found was that the
> spinsystems dialog box allows one to examine and adjust the existing
> sequential segments. Is this the best (or only) way to do this, assuming
> one cannot use the Link Seq Spin Systems function? What would be the
> best way to go about this, either via that menu or otherwise?
You can also RightMouse::Assign::Set sequential spin systems...
T.
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Dr Tim Stevens Email: [log in to unmask]
Department of Biochemistry [log in to unmask]
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