In our original work on a prototype, we used the Cartesian technology. We
were able to dispense 10nL+10nL drops with a large range of viscosities
without difficulty. The main issue was to wash the tips after use to
prevent clogging. That was with a system that could dispense 1nL droplets.
The present systems are sub-nL now. We didn't have a problem with
electrostatics.
More recently, I've looked at all of the crystallization robot vendors.
For single lab users, all of the systems work well. Systems like the Hydra
or Mosquito are less automatic, but provide the basic functions for
crystallization trial setup. For more of a user facility with a large
number of users from several groups, you want more automation to avoid
protocols that can damage the components, like alignment or breakage of
the needles. You also want to consider the total annual cost of
expendables and maintenance.
Bernie Santarsiero
On Wed, January 16, 2008 5:46 am, Demetres D. Leonidas wrote:
> Oryxnano 50+50 nL
>
> Demetres
>
>
> David Briggs wrote:
>> I'll defend the honour of the phoenix... (again)
>> Bernhard Rupp 100+100 nl
>> Dave Briggs (and all users at Univ of Manchester, UK) 100+100nl Others..
>> Only time we have ANY problems is when the nano dispensing tip gets
clogged. Often a good wash whilst still on the machine will clear the
blockage.
>> Dave
>> --
>> ============================
>> David C. Briggs PhD
>> Father & Crystallographer
>> http://www.dbriggs.talktalk.net <http://www.dbriggs.talktalk.net> AIM
ID: dbassophile
>> ============================
>
> --
> Demetres D. Leonidas, Ph.D.
> Structural Biology & Chemistry Group
> Institute of Organic and Pharmaceutical Chemistry
> The National Hellenic Research Foundation
> 48, Vassileos Constantinou Avenue
> Athens 116 35, Greece
> ==================================================
> Tel. +30 210 7273841 (office)
> +30 210 7273895 (lab)
> Fax. +30 210 7273831
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> URL: http://athena.eie.gr
> ==================================================
>
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