You couldnt have the wrong TLS spans could you - if you have added extra
residues have you included them in the TLS group?
Eleanor
Sun Tang wrote:
> Hi James,
>
> I did check teh B-factors and they are similar to the flanking regions (about 40). The difference density appeared at the later stage of refinement (TLS and restrained in CCP4i).
>
> What do you think and how to do it?
>
> Best,
>
> Sun Tang
>
> James Irving <[log in to unmask]> wrote: Hi Sun,
> I suggest checking that the b-factors for those residues aren't
> unexpectedly high compared to those in the flankinh regions.
> Cheers,
> James
>
>
> On 1/23/08, Sun Tang wrote:
>
>> Hello Everyone,
>>
>> When I refined a structure, I found strong difference density Fo-Fc at 3
>> sigma contour for the for five residues which already have occupancy of 1.
>> The density is continuous and so strong as if I did not put the residues
>> there. Why was that? Can I put greater than 1 occupancy for those residues?
>>
>> Thank you very much for your opinions and suggestions!
>>
>> Best wishes,
>>
>> Sun Tang
>>
>>
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>>
>
>
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