You could stain your crystals directly with Sybr Gold, which will
fluoresce upon binding nucleic acids, or you can visualize both protein
and RNA on a silver-stained SDS-PAGE gel.
Kevin
Rongjin Guan wrote:
> Dear All
>
> I am trying to co-crystallize a protein and dsRNA complex, and
> looking for methods to detect RNA in the crystals. I am thinking
> about using Mass Spectrum, Nanodrop to measure OD280/260, etc, but I
> wonder if ther are some more sensitive and reliable ways.
>
> The crystals are grown in high concentration salts; the size is
> usually small but I do have several big ones which can be easily
> fished out. They can be dyed so definitely not salt crystals.
>
> Thanks
>
> Rongjin Guan
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