Two structures of NADH complexes. One is a flavoenzyme. I'm sure there are
many more.
Pejchal R, Sargeant R, Ludwig ML.
Structures of NADH and CH3-H4folate complexes of Escherichia coli
methylenetetrahydrofolate reductase reveal a spartan strategy for a
ping-pong reaction.
Biochemistry. 2005 Aug 30;44(34):11447-57.
PMID: 16114881 [PubMed - indexed for MEDLINE
Inagaki E, Ohshima N, Takahashi H, Kuroishi C, Yokoyama S, Tahirov TH.
Crystal structure of Thermus thermophilus Delta1-pyrroline-5-carboxylate
dehydrogenase.
J Mol Biol. 2006 Sep 22;362(3):490-501. Epub 2006 Jul 29.
PMID: 16934832 [PubMed - indexed for MEDLINE]
> From: Buz Barstow <[log in to unmask]>
> Reply-To: Buz Barstow <[log in to unmask]>
> Date: Sat, 29 Dec 2007 10:55:32 -0500
> To: <[log in to unmask]>
> Subject: [ccp4bb] Crystallographic Experiment with NADH and a Flavoprotein
>
> Dear All,
>
> I'm planning an experiment to study the oxidation of NADH by a
> flavoprotein at cryogenic temperatures to facilitate collection of X-
> ray diffraction data.
>
> In planning this experiment, I have seen a few obstacles that I am
> looking for help in overcoming.
>
> 1. There are no structures in the PDB that are complexed with NADH or
> NAD2H.
> Has anyone ever attempted to solve a structure complexed with NADH or
> NAD2H, especially at cryogenic temperatures, and if so, what are the
> difficulties? Does NAD+ de-bind from the protein too fast to permit
> data collection?
>
>
> 2. NADH oxidation typically takes less than a second by a flavoprotein
> at room temperature.
> Is there an NADH or NAD2H analog that has a much longer half time for
> oxidation by a flavoprotein, for example tens of minutes, rather than
> tenths of a second, and can this analog still be oxidized at cryogenic
> temperatures, with a reasonable half time, of several hours or so?
>
>
> Thanks! and all the best,
>
> --Buz
|