>Hello,
We are trying to purify an N-terminal His6-tagged protein from E.
coli, and the prep was contaminated with the E coli
Glucosamine-fructose-6-phosphate aminotransferase, which co-purifies
with my protein of interest in subsequent ion-exchange and sizing
columns. This protein appears to be a common contamination in the
Ni-NTA purifications. Does anyone have tricks to get rid of it?
Thanks.
Ailong
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