> In one case, for example, cleaving the N-terminal His-tag improved
> the solubility of the protein dramatically. Interestingly,
> expression levels of the protein without the His-tag were much
> lower than with it!
That is also quite common ;-) Look at the second and third codons:
optimizing these (as they are optimized in His-tag vectors) can
dramatically enhance expression levels.
A.
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