It did not contain DTT when frozen.
On 11/13/07, deena <[log in to unmask]> wrote:
> Are you sure its 1mM DTT, because DTT itself precipitates in the freezer.
>
> Deena
>
>
> On Nov 13, 2007, at 3:30 PM, Joe wrote:
>
> Hi there,
> I see enormous precipitate of my receptor protein when I take it out
> of freezer. But all the precipitate dissolved quickly after I added
> 1mM DTT to the solution. Does this mean that some surface Cys are
> causing problem? Or why is the protein so sensitive to DTT?
> Anybody experienced this kind?
> Any advice is appreciated.
> -Joe
>
> Deena Abells Oren, PhD
> Manager, Structural Biology Resource Center
> Rockefeller University
> 1230 York Ave
> New York, NY 10065-6399
> phone: 212-327-7429
>
>
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