This may not apply in your case but it is not uncommon for a protein to
"precipitate" in a microcrystalline shower when put in cold. Once it
worms up, the crystals dissolve and the precipitate clears up. It is
easy to check under a high magnification microscope.
Cheers,
N.
Ruslan Sanishvili (Nukri), Ph.D.
GM/CA-CAT, Bld. 436, D007
Biosciences Division, ANL
9700 S. Cass Ave.
Argonne, IL 60439
Tel: (630)252-0665
Fax: (630)252-0667
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-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
Joe
Sent: Tuesday, November 13, 2007 2:30 PM
To: [log in to unmask]
Subject: [ccp4bb] DTT sensitive?
Hi there,
I see enormous precipitate of my receptor protein when I take it out of
freezer. But all the precipitate dissolved quickly after I added 1mM DTT
to the solution. Does this mean that some surface Cys are causing
problem? Or why is the protein so sensitive to DTT?
Anybody experienced this kind?
Any advice is appreciated.
-Joe
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