Dont forget pointless gives a point group so you could have spacegroup
P4123 too - check that in MR
Could might well have a trimer sitting on the cubic 3-fold, but then the
oligimer will be generated by the crystal symmetry, and you need only
search with a monomer.
Eleanor
Kristof Van Hecke wrote:
> Dear all,
>
> When running Pointless 1.2.0 (see summary below), I get the cubic
> space group P 4 3 2
> (mosflm gave P 2 3 or P 4 3 2)
>
> <!--SUMMARY_BEGIN-->
>
> Best Solution space group P 4 3 2
> Reindex operator: [h,k,l]
> Laue group probability: 1.000
> Systematic absence probability: 0.975
> Total probability: 0.975
> Space group confidence: 0.964
> Laue group confidence 1.000
>
> <!--SUMMARY_END-->
>
> However, although I have multiple models (ensemble) with approximately
> 30% sequence identity (44% homology), I'm not able to get any
> reasonable solution with Phaser.
> As we know the protein can form dimers and even trimers, is it
> possible that Poinless is giving a higher space group because of
> pseudo-symmetry in this case..?
>
> Does anyone has got experience with Pointless and this kind of space
> group please..?
>
> Many thanks
>
> Kristof
>
> --------------------------------------
> Kristof Van Hecke, PhD
> Biomoleculaire Architectuur
> Celestijnenlaan 200 F
> B-3001 Heverlee (Leuven)
> Tel: +32(0)16327477
> --------------------------------------
>
>
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>
>
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