Did you check the pH of your DTT and the protein solution before and after?
I have had some experience with DTT changing pH (as might well be
expected...).
Jacob
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Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.467.4049
cel: 773.608.9185
email: [log in to unmask]
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----- Original Message -----
From: "Joe" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Tuesday, November 13, 2007 2:30 PM
Subject: [ccp4bb] DTT sensitive?
> Hi there,
> I see enormous precipitate of my receptor protein when I take it out
> of freezer. But all the precipitate dissolved quickly after I added
> 1mM DTT to the solution. Does this mean that some surface Cys are
> causing problem? Or why is the protein so sensitive to DTT?
> Anybody experienced this kind?
> Any advice is appreciated.
> -Joe
>
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