Dear all,
Recently, I crystallized a mutant protein in which a Glycine was changed to Proline. The resolution of the crystal is 3.35A. I performed molecular replacement and refinement with CCP4. Surprisingly, no electronic density can be detected for the side chain of Proline. Since Proline is always considered rigid, I wonder if it is possible that this Proline is so flexible that its map becomes undetectable? The density map is attached.
Thanks in advance!
Tiancen Hu
Shanghai Institute of Materia Medica
Rm. 2107, #555, ZuChongzhi Rd.
Shanghai 201203
P.R. China
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