Yes to keeping the same FreeR
Bfactors - I doubt if it matters, but I usually start a new data set by
setting Bs to the Wilson suggestion..
Twinned data - maybe you ought to use SHELXL to refine it?
Eleanor
john kryst wrote:
> Hi all !!
>
> I am working on some deletion mutants. Mutants crystallized in
> the same space group with similar cell dimensions. My questions are the
> following??
>
> 1. Is the free R flag has to be maintained across all the mutants with
> respect to the native.
> 2. Does B-factor has to be flattened (say 30) before starting the
> refinement of the each mutant.
> 3. One of my mutant is twinned. should i maintain the same free R flag
> without worrying about the twinning ???
>
> Thanks for your inputs.
>
> with warm regards
> John
>
>
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