I'm currently assigning HNCA and HNcoCA using AutoAssign and the CCPNMR Link
Seq Spin System tool. I've used the Link Peak Lists tool to assign peaks in
my 3D spectra from the HSQC. I've imported assignments from AutoAssign, and
my problem now is that the carbon-shift in my HNCA and HNcoCA spectra is not
assigned, even when the spin systems are linked. Shouldn't CCPNMR do the
automatically?
In my HNCA the i-1 peak is called: "16ValH - 16ValN" and the i peak is
called "16ValH 16Val[468] 16ValN". In my HNcoCA the peak corresponding peak
is called "16ValH 15Glu[467] 16ValN". Is it because of the AutoAssign import
that this isn't working correctly, or are you supposed to assign CA-shifts
yourself?
Robert
|