Tim,
Thanks for your help. I have not used the "Set Seq Link." Do you mind briefly explaining what this does in terms of the resonance and spin systems?
If I understand correctly, using the Link Sequential Spin Systems to set the i HNCA resonances to the same spin system and to set the other as i-1 makes for a catch-22 situation, especially considering the exchange between Justin and Wim: where spin systems have to be set for format converter to export to a certain format.
Thanks again,
Mike
Michael Latham
Graduate Student
Department of Chemistry and Biochemistry
University of Colorado, Boulder
Phone: 303-492-8085
Fax: 303-492-2439
---- Original message ----
>Date: Thu, 25 Oct 2007 14:16:55 +0100
>From: Tim Stevens <[log in to unmask]>
>Subject: Re: Sequential Assignments
>To: [log in to unmask]
>
>> > The 13C resonance of the i-1 peak in the HNCA and the corresponding peak
>> > in the HN(CO)CA have different resonance numbers, and they should be the
>> > same. Also, the 13C resonance of the i peak in the HNCA does not belong
>> > to the same spin system as the 1H and 15N resonance. Presumably, this
>> > was done because a priori we do not know which of the HNCA 13C peaks is
>> > i or i-1, but with the HN(CO)CA we do. So I guess the second question
>> > here is can this process be streamlined (or did I miss something in an
>> > earlier step)?
>>
>> Oh bum. After testing I see that things have become broken. What is
>> supposed to happen (and indeed what used to happen) is that the spin
>> systems and resonances are merged so that they are all consistent once you
>> set the sequential link. I shall attempt to fix...
>
>Oops. After looking at the correct peak this time I see that things are
>not broken. The resonances that match in carbon shift, and their spin
>systems are merged. Did you use "Set Seq Link"?
>
>T.
>
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> Department of Biochemistry [log in to unmask]
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>------ +NH3CH(CH(CH3)OH)C(O)NHCH(CH(CH3)CH2CH3)C(O)NHCH(CH2CH2SCH3)CO2- -------
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