> 1) Is there an easy way to merge resonances?
Hi Michael,
Yes, you can do it from both the Edit Assignment popup (when they are
assigned to the same peak dimension) and via the Browse Resonances popup:
select two and hit [Merge].
> The 13C resonance of the i-1 peak in the HNCA and the corresponding peak
> in the HN(CO)CA have different resonance numbers, and they should be the
> same. Also, the 13C resonance of the i peak in the HNCA does not belong
> to the same spin system as the 1H and 15N resonance. Presumably, this
> was done because a priori we do not know which of the HNCA 13C peaks is
> i or i-1, but with the HN(CO)CA we do. So I guess the second question
> here is can this process be streamlined (or did I miss something in an
> earlier step)?
Oh bum. After testing I see that things have become broken. What is
supposed to happen (and indeed what used to happen) is that the spin
systems and resonances are merged so that they are all consistent once you
set the sequential link. I shall attempt to fix...
> 2) Next is more of a problem than question. When I run the Link
> Sequential Spin Systems utility, set up as described in the tutorial, I
> get what is expected and shown in the first screen shot (LSS_1 rainbow
> contours HN(CO)CA and blue contour HNCA). However, if I turn the
> HN(CO)CA back on in the matches window, it can be seen that strips 3,7,9
> and 10 should be omitted because they are i-1 peaks in the HNCA not i
> (LSS_2). The experiments are setup correctly.
The system simply does not filter these possibilities; that is still left
to the user, so I would advise leaving the HN[co[CA]] on.
This system might get some more development time in the future, but I
deliberately stopped making it more fancy because there is a more
holistic and automatic system coming on line soon: Somewhat like MARS or
Autoassign, but with everything tied into the Analysis
windows/strips/tables.
T.
-------------------------------------------------------------------------------
Dr Tim Stevens Email: [log in to unmask]
Department of Biochemistry [log in to unmask]
University of Cambridge Phone: +44 1223 766018 (office)
80 Tennis Court Road +44 7816 338275 (mobile)
Old Addenbrooke's Site +44 1223 364613 (home)
Cambridge CB2 1GA WWWeb: http://www.bio.cam.ac.uk/~tjs23
United Kingdom http://www.pantonia.co.uk
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